Probing the polar metabolome by UHPLC-MS

Metabolomics is an interdisciplinary field with applications in many areas. Analytes include metabolites involved pathways such as glycolysis, amino acid metabolism, the Krebs cycle, etc. However, these biosynthetic are typically highly polar molecules, which represent a major challenge for chromatographic analysis. Whilst there have been significant efforts to address difficulties determination of comprehensive profiling metabolome remains problematic. The current review summarizes approaches, advances and trends use liquid chromatography/mass spectrometry it attempts instrumental intellectual challenges mapping metabolome.